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What is the refractive index of RapiClear??
The number labeled alongside with RapiClear represents the corresponding refractive index (RI). For example, the RI of RapiClear 1.52 is 1.52nD (589.3 nm).

Will post-fixation pose any additional problem with RapiClear?
Post-fixation is a recommended step after immunohistochemical staining to prevent sample deformation. After thoroughly washed with PBST, post-fixation should not make any side effect for the clearing function of RapiClear.

What is the transmission of RapiClear?
The solution absorbs light at 370nm but is permissive to light greater than 400 nm

I have some problems applying RapiClear to thick samples. Why I only got blurred images after treatment?
For tissues thicker than 0.3mm, we suggest that fluorescence dye with near infrared spectrum, such as Alexa 635 or 647 should be applied.
In addition, it is important to use objective lens with high NA as well as long working distance to capture images (e. g. Leica HCX IRAPOL 25X water-immersion objective, NA 0.95, WD 2.4mm; Zeiss 20X W-PlanApochromat objective, NA 1.0, WD 2.0 mm; and Olympus XLSLPLN25XSVMP objective, NA 0.9, WD 8.0 mm).

Can I get information about the compatibility of organic dyes with RapiClear?
Based on our trial results, the Alexa FluorR Dyes and CyDye Reactive Fluorescent Dyes are perfectly compatible with RapiClear.


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