FluoTissue® prepared slide contains a 550μm fixed tissue section cleared and mounted in RapiClear® 1.52 reagent (Cat. no. RC152001/RC152002). RapiClear® 1.52 is an aqueous tissue clearing reagent not only can highly preserve tissue components, but matches the refractive indices of fixed tissue section to that of immersion oil and glass coverslips (1.52nD) to minimize both light scattering and spherical aberrations. Each section multi-labeled with fluorescent dyes is especially useful for confocal microscopy to create stunningly high-quality images and reconstruct 3D models in sub-micrometer-scale resolution.
Spectral characteristics of the dyes labeled in FluoTissue® prepared slide.
Alexa Fluor® 488
Alexa Fluor® 633
The video shows the microstructures of the mouse brain, with Alexa Fluor 488- WGA labels the blood vessels in green, SYTOX Orange labels the nuclei in cyan, and Alexa Fluor 633 anti-TH antibody mainly labels the dopaminergic neurons.