Information
FluoTissue® prepared slide contains a 550μm fixed tissue section cleared and mounted in RapiClear® 1.52 reagent (Cat. no. RC152001/RC152002). RapiClear® 1.52 is an aqueous tissue clearing reagent not only can highly preserve tissue components but matches the refractive indices of fixed tissue section to that of immersion oil and glass coverslips (1.52nD) to minimize both light scattering and spherical aberrations. Each section multi-labeled with fluorescent dyes is especially useful for confocal microscopy to create stunningly high-quality images and reconstruct 3D models in sub-micrometer-scale resolution.
FluoTissue selection guide
Spectral characteristics of the dyes labeled in FluoTissue® prepared slide.
Dyes | Excitation max. | Emission max. |
Alexa Fluor® 488 | 496 nm | 519 nm |
SYTOX® Orange | 547 nm | 570 nm |
Alexa Fluor® 647 | 650 nm | 665 nm |
Video
The video shows the microstructures of the mouse kidney, with Alexa Fluor 488 labels the blood vessels in green, SYTOX Orange labels the nuclei in cyan, and Alexa Fluor 647- TUJ1 antibody mainly labels the nervous system.
The video shows the microstructures of the mouse intestine, with Alexa Fluor 488 labels the blood vessels in green, SYTOX Orange labels the nuclei in cyan, and Alexa Fluor 647- LYVE1 antibody mainly labels the lymphatic vessels.
The video shows the microstructures of the mouse brain, with Alexa Fluor 488 labels the blood vessels in green, SYTOX Orange labels the nuclei in cyan, and Alexa Fluor 647- TH antibody mainly labels the dopaminergic neurons.
The video shows the microstructures of the mouse liver, with Alexa Fluor 488 labels the blood vessels in green, SYTOX Orange labels the nuclei in cyan, and Alexa Fluor 647- TUJ1 antibody mainly labels the nervous system.